Solid phase extraction technology of solid phase extraction device

Solid-phase extraction technology of the LNB brand solid-phase extraction device developed and produced by Shanghai Xinzhuang Instrument Co., Ltd. - Since the solid-phase extraction method was introduced in the late 1970s, the development of various SPE columns with different phases has been rapidly developed. The solid phase extraction method is continuously adapted to the needs of pretreatment of various samples, and the solid phase extraction method for different types of compounds is gradually improved.

Solid phase extraction (Solid Phase Extraction) is the use of solid adsorbent to adsorb the target compounds in liquid samples, and the matrix and interference compounds of the sample separation, and then eluted with eluent or desorbed by heating, to achieve separation and enrichment targets The purpose of the compound. Solid-phase extraction as a sample pretreatment technology has been more and more widely used in the laboratory.

SPE is a column chromatographic separation process. There are many similarities between high performance liquid chromatography (HPLC) and the separation mechanism, stationary phase and solvent selection. However, the SPE column has a larger particle size (>40 μm) than the HPLC packing (3 to 10 μm). Due to the short column bed and large particle size, SPE column efficiency is much lower than HPLC column. A high-efficiency HPLC column can produce more than 10,000 plates, while a SPE column can obtain 10 to 50 plates. Therefore, compounds with very different properties are retained separately by SPE. In this case, the typical SPE separation is performed not in an isocratic manner but in a digitaIon-offmode. This means that with a stepped gradient, the analyte is either not adsorbed firmly by the stationary phase or is not retained at all. Another difference with HPLC is that the SPE column is for single use.

The characteristics of the SPE column itself determine its application range. The SPE technology with lower separation efficiency is mainly used to process samples. The objectives to be achieved with SPE are: (1) to remove interfering substances from the sample for subsequent analysis; (2) to enrich trace components and to increase analytical sensitivity; (3) to convert the sample solvent to analysis The methods are matched; (4) In-situ derivatization; (5) Desalting of the sample; (6) Convenient storage and transportation of the sample.

Among the above points, purification and enrichment are the most important.

More about solid-phase extraction technology consulting, please consult Shanghai Xinzhuang Instrument Co., Ltd., Shanghai Xinzhuang Instrument Co., Ltd. Senior Engineer will answer for you, Shanghai Xinzhuang Instrument Co., Ltd. independent research and development of Xinzhuang (LNB) brand Solid phase extraction device products are widely used, high stability, quality guaranteed, welcome new and old customers to consult and buy!