Principle of ELISA experiment of human leukocyte common antigen (LCA / CD45)

2024-05-15 09:07:40

1. The level of human leukocyte common antigen (LCA / CD45) ELISA Kit was determined by the double antibody sandwich method.

2. First coat the microplate with purified human leukocyte common antigen (LCA / CD45) ELISA Kit antibody to make a solid phase antibody.

3. Then add the leukocyte common antigen (LCA / CD45) ELISA Kit to the microwells coated with mAb in turn, and then combine with the HRP-labeled leukocyte common antigen (LCA / CD45) ELISA Kit antibody to form an antibody-antigen-enzyme labeled antibody After thorough washing, the complex is added with substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.

4. The color depth is positively correlated with the white blood cell common antigen (LCA / CD45) ELISA Kit in the sample.

5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of the human leukocyte common antigen (LCA / CD45) ELISA Kit in the sample is calculated by the standard curve

Objective: To determine the content of leukocyte common antigen (LCA / CD45) ELISA Kit in human serum, plasma and related liquid samples.

6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.

7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.

safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.

4 Keep Elisa kit away from children

Bring your own items:

1. 37 â„ƒ incubator

2. Standard specification microplate reader

3. Precision pipette and disposable tip

4. Distilled water

5. Disposable test tube

6. Absorbent paper

Storage conditions and validity period:

1. Kit storage :; 2-8 â„ƒ.

2. Validity: 6 months